Wilson Wolf G-Rex底透氣型細胞培養瓶
——封閉式免疫細胞培養，CAR-T、CAR-NK、TCR-T、TIL細胞GMP生產

 
北京澤平代理的美國Wilson Wolf G-Rex底透氣型細胞培養瓶，針對懸浮細胞培養設計，實現細胞快速擴增和封閉條件下收獲，特別適用于免疫細胞的實驗室研究和大規模GMP生產，目前已成功用于包括CAR-T、CAR-NK、TCR-T在內的多種工程細胞的封閉式培養中：

①免疫細胞培養
嵌合抗原受體T細胞（CAR-T，chimeric antigen receptor T cells）
嵌合抗原受體NK細胞（CAR-NK，chimeric antigen receptor natural killer cells）
T細胞受體嵌合型T細胞（TCR-T，T cell receptor-engineered T cells）
EB病毒特異性細胞毒性T淋巴細胞（EBV-CTL）
腫瘤浸潤淋巴細胞（TIL細胞，tumor infiltrating lymphocytes）
自然殺傷細胞（NK細胞，natural killer cells）
調節性T細胞（Treg細胞，regulatory T cells）
造血干細胞（HSC，hematopoietic stem cell）
淋巴母細胞系（LCL，lymphoblastoid cell line）
抗原特異性T細胞（CTL）
人慢性骨髓性白血病細胞系（K562細胞系）

②雜交瘤、CHO、293等單克隆抗體和重組抗體生產
③多至40001E/cm2的膜島運輸
 





參考文獻

1. Optimlzation of therapeutic T cell expansion in G-Rex device and applicabillty to large-scale production for clinical use. Gotti, et al. Cytotherapy, 1/19/2022
2. Rapid Transduction and Expansion of Transduced T Cells with Maintenance of Central Memory Populations. Mary Pampusch, Kumudhini Haran, Geoffrey Hart, et al. Molecular Therapy Methods & Clinical Development, 3/1/2020
3. Innovative Development of Closed CAR T Platform(Poster). Gil Joseph, et al. WuXi Advanced Therapies, 1/1/2020
4. A serum-free protocol for the ex vivo expanslon of Cytokine-Induced Killer cells using gas-permeable static culture flasks. Palmerini, et al. Cytotherapy, 9/1/2020
5. Streamlined production of genetically modified T cells with activation, transduction and expanslon in closed-system G-Rex bloreactors. Christine Gagliardi, et al. Cytotherapy, 10/16/2019
6. Enhanced clinical-scale manufacturing of TCR transduced T-cells using closed culture system modules. Jianjian Jin, et al. Journal of Translational Medicine, 1/1/2018
7. Tumor-infiltrating lymphocytes: Streamlining a complex manufacturing process. Emily Hopewell, et al. Cryotherapy, 11/4/2018
8. Large-scale expansion ofVγ9Vδ2T cells with engineered K562 feeder cells in G-Rex vessels and their use as chimerlc antigen receptor-modified effector cells. Lin Xiao, et al. Cytotherapy, 12/17/2017
9. Large-Scale Culture and Genetic Modification of Human Natural Killer Cells for Cellular Therapy. Natalia Lapteva, et al. Methods in Microbiology, 1/1/2016
10. The beneficlal effects of a gas-permeableflask for expansion of Tumor-Infiltrating lymphocytes as reflected in their mitochondrlal function and respiration capacity. Marie-Andree Forget, et al. Oncolmmunology, 3/2/2016
11. Manufacture of GMP-compllant functional adenovirus-specificT-cell therapy for treatment of post-transplant infectlous complications. Claire Horlock, et al. Cytotherapy, 6/1/2016
12. Go-CART: an animal-free system for the assessment of CAR T cell function. Pradip Bajgain, et al. Journal of Immunotherapy of Cancer, 11/4/2015
13. Manufacture of tumor-and virus-speclific T lymphocytes for adoptive cell theraples. XWang, et al. Cancer Gene Therapy, 2/27/2015
14. Early transduction produces highly functional chimeric antigen receptor-modified virus-specific T-cells with central memory markers: a Production Assistant for Cell Therapy(PACT) translational application. Jiali Sun, et al. Journal of Immunotherapy of Cancer, 1/1/2015


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——Wilson Wolf官方授權中國代理商，北京澤平