1604 次Fluorescent Staining of Cells 2012-6-20
1. Fluorescent phalloidin in methanol. Phallacidin does not work as well. Dilute 10 ul 330 nM stock into 500 ul PBS for each large coverslip. 2. PBS, solution A. Procedure:1. Fix a
2248 次Placental trophoblast and chorionic cell cultures 2011-12-16
Placental trophoblast and chorionic cell cultures 1.Placental trophoblast and chorionic trophoblast cells were prepared using a modification of the method. 2.Term human placentae a
1795 次Isolation of dermal mast cell (DMC) 2011-9-22
Isolation of dermal mast cell (DMC) 1.Human DMC were isolated from skin by enzymatic digestion.2.Dermal tissue was obtained from patients with skin after informed consent was give
9222 次Isolation of retinal pigment epithelial cell 2011-9-16
Isolation of retinal pigment epithelial cell The pigmented layer of retina or retinal pigment epithelium (RPE) is the pigmented cell layer just outside the neurosensory retina that
2259 次Primary brain cell isolation and culture 2011-6-13
Primary brain cell isolation and culture. 1.Cerebella were removed from 7-day-old mice and passed through Nitex nylon netting (80 μm pore size) into primary cell system containin
15054 次Isolation and Culture of Human Brain Tumor Stem Cell 2011-4-1
Isolation and Culture of Human Brain Tumor Stem CellThe isolation, culture, identification, and purification of stem cells from primary human brain tumors of different phenotypes h