Figure 2. Comparison of GFP expression profile in two platforms

U-2 OS cells were transfected using the Neon™ transfection system (from Life Technologies) and 0.25 µg of the five different GFP constructs and then analyzed on the Arthur™ and competitor’s product F to determine transfection efficiency. The histograms in panels A (Arthur™) and C (competitor’s product F) show the green fluorescence intensities  and counts for the transfected populations. The percentages of GFP-expressing cells detected with the Arthur™ were consistent with the results from the competitor’s product F. ‘Colored circles’ option makes it easy to identify gated cells (Panel B); colored circles represent: cells expressing GFP (green circles), non-GFP-expressing cells (blue circles). The image in panel D shows the GFP expressing cells before trypsinization under the conventional fluorescence microscope.

Figure 4. Comparison of cell cycle profiles in two platforms:
Arthur™ and Competitor’s product F

HeLa cells were treated with Nocodazole (microtubule-destabilizer) or Epothilone B
(microtubule-stabilizer) to arrest cells in G2/M phase. Cells were fixed with 70%
ethanol and stained with Propidium Iodide(PI). The histograms show the percentages
of G0/G1 phase, S phase and G2/M phase of cell cycle analysis for drug treated
HeLa cells on the Arthur™ (A) and Competitor’s product F (B).
The cell cycle analysis of Arthur™ was highly consistent with the results obtained
from competitor’s product F.

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