規(guī)格: 30 µL |
基因符號: Gna13 |
描述: Anti-Gα13-GTP Mouse Monoclonal Antibody |
背景介紹: Heterotrimeric G proteins are essential cellular signal transducers. Gα13 is one of the G proteins that could mediate cell migration and angiogenesis. Other biochemical and physiological functions of Gα13 are being explored. |
免疫原: Recombinant full length active Gα13 protein |
經(jīng)過測試的應(yīng)用: IP, IHC, IF |
推薦稀釋比:
IP: 1 µg for 1~2 mg total cellular proteins
IHC, IF: 1:50-1:250 |
濃度: 1 mg/ml |
宿主: Mouse |
形式: Liquid |
克隆性: Monoclonal |
亞型: IgG2b |
純度: Purified from ascites |
防腐劑: No |
成分: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol |
種屬反應(yīng)性: Anti-active Gα13 monoclonal antibody recognizes active Gα13 of vertebrates. |
儲存條件: Store at -20°C. Avoid repeated freezing and thawing |
免疫沉淀/蛋白質(zhì)印跡:
IP/WB analysis of active Gα13 proteins. Purified Gα13 proteins were treated with GDP (lane 1) or GTPGTPγS (lane 2). After immunoprecipitation with anti-active Gα13-GTP mouse monoclonal antibody (Cat. # 26902), these proteins were separated by SDS/PAGE and western blotted with anti- Gα13 mouse monoclonal antibody (Cat. # 26004). |
Immunohistochemistry:
Staining of MEF cells (treated with PDGF) with anti- Gα13-GTP monoclonal antibody showing the localization of Gα13-GTP in the leading edge of migrating cells. |