• Molecular Biology/分子生物學
  • Plasmid DNA preparation/質粒制備
  • Subcloning/亞克隆
  • Custom ORF cloning/ORF克隆
  • Mutagenesis services/基因突變
  • Molecular diagnosis/分子診斷

• Protein Production Systems and Purification /蛋白質表達系統以及純化

• The E. Coli System/大腸桿菌表達系統 

                  ○ Generation of Recombinant Plasmid /重組質粒構建  

                  ○  Expression Test in Host /重組表達測試

                  ○ Soluble Expression Test / 可溶性表達條件測試  

                  ○ Small Scale Expression and Purification (2L Cell Culture)/小規模蛋白表 達  和純化（2L培養基培養產物） 

                  ○  Scale Up Expression and Purification/大規模蛋白表達和純化（10L以上）

• The Baculovirus System/昆蟲細胞表達系統

                 ○  重組桿粒（bacmid DNA）制備/Generation of recombinant Bacmid DNA

                 ○ 重組桿狀病毒制備/Preparation of recombinant baculovirus

                 ○  重組病毒滴度測定/Titration of baculovirus

                 ○  重組蛋白表達條件優化/Protein expression verification and optimization

                 ○  小規模蛋白表達和純化（500 ml培養物）/Small scale expression and purification of recombinant protein in insect cell

                 ○ 大規模蛋白表達和純化（可選）/Scale up expression and purification of recombinant protein in insect cell

• The Mammalian System/哺乳動物表達系統
  • CHO, Vero, BHK, 293 cells etc/CHO, Vero, BHK, HEK293 等多種表達系統
  • Adherence and suspension culture/貼壁及懸浮培養      
  • Transient expression screen/瞬轉表達篩選
  • Stable cell line generation/穩定細胞系建立

Recombinant protein expression and purification Services/酵母表達系

          ○  重組酵母表達質粒構建/Generation of Recombinant Plasmid

          ○ 重組質粒電轉化/Expression Test in Host

          ○ 重組表達子篩選/Soluble Expression Test

          ○ 小規模蛋白表達和純化（2L培養基培養產物）/Small Scale Expression and Purification (2L Cell Culture)

          ○ 大規模蛋白表達和純化（10L以上）/Scale Up Expression and Purification

• Computational Biology & Molecular Modeling/計算生物學和分子模型構建
  • Structural-Based Drug Design (SBDD)/基于結構的藥物設計
    • Target family analysis: using prior knowledge (specificity)/目標族群分析：利用優先知識（特異性）
    • Homology modeling/同源建模
• Quantitative structure-activity relationship (QSAR)/定量構效關系
• Virtual drug screening/計算機虛擬篩選
  • Pharmacophore match for docking-based virtual screen/藥物匹配對接的虛擬篩選
  • • Hypothesis-driven docking/假設驅動的對接
    • Enhancing results of virtual screens using data mining/提高使用數據挖掘的虛擬篩選的結果
    • De novo drug design/全新藥物設計

詳情請查看美迪西官網www.medicilon.com.cn