Preparation :

Produced in mice using highly pure (>98%) recombinant human soluble IL-2 Receptor alpha as the immunizing antigen. This IgG2_aK antibody was purified from cell culture by Protein G affinity chromatography.

Catalog #:

500-M02R

Source :

Mice

Formulation :

A sterile filtered antibody solution was lyophilized from PBS.

Stability :

The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20^o C. Frozen aliquots are stable for at least six weeks at 2-8^o C or for at least six months at -20^o C. Avoid repeated freeze-thaw cycles.

Reconstitution:

Centrifuge vial prior to opening. Reconstitute in sterile water to a concentration of 1.0 mg/ml.

Applications :

Neutralization 

To yield one-half maximal inhibition [ND ₅₀ ] of the biological activity of hsIL-2Rα (10.0 μg/ml), a concentration of 1.0-1.5 µg/ml of this antibody is required, in the presence of 0.5 ng/ml recombinant hIL-2.

Elisa 

In a sandwich ELISA (assuming 100µl/well), a concentration of 4.0-8.0 µg/ml of this antibody will detect at least 100 pg/ml of recombinant human sIL-2Rα when used in conjunction with compatible secondary reagents.

Western Blot 

To detect hsIL-2Rα by Western Blot analysis this antibody can be used at a concentration of 0.20-0.40 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hsIL-2Rα is 2.0-4.0 ng/lane, under reducing or non-reducing conditions.

Country of Origin :

USA

Special Sizes:
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Usage:
For Research Use Only. Not for use in diagnostic or therapeutic procedures.