Preparation :
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Produced from sera of goats pre-immunized with highly pure (>98%) recombinanthIL-6. Anti-Human IL-6 specific antibody was purified by affinity chromatography employing immobilized hIL-6 matrix.
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Catalog #:
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500-P26G
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Source :
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Goats
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Formulation :
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A sterile filtered antibody solution was lyophilized from PBS, pH 7.2.
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Stability :
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The lyophilized antibody is stable for at least 2 years from date of receipt at -200
C. The reconstituted antibody is stable for at least two weeks at 2-80
C. Frozen aliquots are stable for at least 6 months when stored at -200
C. Avoid repeated freeze-thaw cycles.
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Reconstitution:
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Centrifuge vial prior to opening. Reconstitute in sterile water to a concentration of 0.1-1.0 mg/ml.
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Applications :
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Neutralization
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To yield one-half maximal inhibition [ND
50
] of the biological activity of hIL-6 (5 ng/ml), a concentration of 0.05 - 0.08 µg/ml of this antibody is required.
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Elisa
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Indirect
:To detect hIL-6 by indirect ELISA (using 100 μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hIL-6.
Sandwich
:To detect hIL-6 by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with ’s Biotinylated Anti-Human IL-6 (500-P26GBt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hIL-6.
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Western Blot
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To detect hIL-6 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIL-6 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
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Country of Origin :
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USA
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| Size A
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50µg
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$195
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| Size B
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100µg
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$250
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| Size C
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1mg
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$2100
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Special Sizes:
Many products can be ordered in custom sizes. Please click here
to contact with your request.
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Usage:
For Research Use Only. Not for use in diagnostic or therapeutic procedures.
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