Preparation :

Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant mCTLA-4. Anti-Murine CTLA-4 specific antibody was purified by affinity chromatography employing immobilized mCTLA-4 matrix.

Catalog #:

500-P147

Source :

Rabbits

Formulation :

A sterile filtered antibody solution was lyophilized from PBS, pH 7.2.

Stability :

The lyophilized antibody is stable for at least 2 years from date of receipt at -20⁰ C. The reconstituted antibody is stable for at least two weeks at 2-8⁰ C. Frozen aliquots are stable for at least 6 months when stored at -20⁰ C. Avoid repeated freeze-thaw cycles.

Reconstitution:

Centrifuge vial prior to opening. Reconstitute in sterile water to a concentration of 0.1-1.0 mg/ml.

Applications :

Neutralization 

Data not available

Elisa 

Indirect :To detect mCTLA-4 by indirect ELISA (using 100 μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2 - 0.4 ng/well of recombinant mCTLA-4.
Sandwich :To detect mCTLA-4 by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with ’s Biotinylated Anti-Murine CTLA-4 (500-P147Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant mCTLA-4.

Western Blot 

To detect mCTLA-4 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant mCTLA-4 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

Country of Origin :

USA

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Usage:
For Research Use Only. Not for use in diagnostic or therapeutic procedures.