Synonyms :

Leukocyte interferon, B cell interferon, Type I interferon

Description :

Proteins of this family play an important role in inducing non-specific resistance against a broad range of viral infections. They also affect cell proliferation and modulate immune responses. Produced by peripheral blood leukocytes and lymphoblastoid cells, IFNα is an acid stable molecule that signals through IFNα/βR, which is also used by IFNβ. Both IFNs have similar anti-viral activity and regulate expression of MHC class I antigens. IFNα contains four highly conserved cysteine residues which form two disulfide bonds, one of which is necessary for biological activity. Recombinant human IFNβ is an 20.0 kDa protein containing 166 amino acid residues. Due to glycosylation, IFNβ has an approximate MW of 22.3 kDa based on SDS-PAGE gel and Mass Spectrometry.

*Human IFN-beta(Catalog Number 300-02BC) has replaced Human IFN-beta(Catalog Number 300-02B)

Catalog #:

300-02BC

Source :

CHO cells

Purity :

Greater than 95% by SDS-PAGE gel and HPLC analyses.

Endotoxin Level :

Endotoxin level is less than 0.1 ng per µg (1EU/µg).

Biological Activity :

Assay#1: The biological activity of 's human IFN-β was based on analysis using the WISH cell line with VSV as the challenge virus. The specific activity was determined to be >5 x 10 ⁸ units/mg
Assay#2: Measured by its ability to induce apoptosis in HeLa cells. The expected ED ₅₀ for this effect is 20-30 ng/ml.

Selected References :

Selected List of References Citing This Product

AA Sequence :

MSYNLLGFLQ RSSNFQCQKL LWQLNGRLEY CLKDRMNFDI PEEIKQLQQF QKEDAALTIY EMLQNIFAIF RQDSSSTGWN ETIVENLLAN VYHQINHLKT VLEEKLEKED FTRGKLMSSL HLKRYYGRIL HYLKAKEYSH CAWTIVRVEI LRNFYFINRL TGYLRN

PubMed Link :

IFN-beta

Country of Origin :

USA

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Usage:
For Research Use Only. Not for use in diagnostic or therapeutic procedures.