高通量單細胞力學熒光測試分析系統將細胞生物力學特性同流式細胞儀有機的結合起來，能夠在單細胞水平下，高通量、無生物標記物的條件下，快速研究細胞的生物力學特性。

為了給細胞加載力學刺激，單個細胞被泵驅動通過橫截面略大于細胞的橫截面的微通道。細胞周圍的流體的壓力梯度創造出一個流動剖面而影響細胞的水動力學。通過流體的流速和粘度可控制作用于細胞上的力。
細胞可以通過水動力可使細胞發生形變；
力由流體的流速和粘度控制；
軟細胞能展示出更大程度的變形。

產品配置列表
高通量單細胞力學特性分析模塊
包括：
 - 高速成像系統（CMOS相機和圖像采集卡）
 - 大功率LED照明系統（460 nm）
 - 帶兩個注射器模塊的精密注射泵
 - 泵架，SampleStage和SampleBox，
 - 高性能計算機
 - 控制和采集軟件（單機許可證，附帶許可協議）
- 用于后期處理的數據可視化軟件
倒置顯微鏡 - 蔡司Axio Observer 3
和高通量單細胞力學特性分析模塊一起進行“實時高通量細胞力學性能測試”。
包含：
 - 左側端口，與高速相機實現高速通信
 -  40x物鏡，NA 0.65，空氣/無浸泡
 - 手動XY平臺
2.1 附件選項1：升級到自動 XY 平臺
2.2附件選項2：升級到 Axio Observer 7 和自動XY 平臺
保溫模塊（選配）
與蔡司 Axio Observer兼容，加熱高通量單細胞力學特性分析模塊，控制樣品在測試期間的溫度在室溫和37°C之間。
熒光模塊（選配）
在單細胞力學測量同時，進行熒光強度測量。
與蔡司Axio Observer顯微鏡兼容，激光發射模塊直接接到顯微鏡側面端口上。 
可選擇的激發波長和檢測通道如下：
3.1 激發波長 488nm，用于FITC，GFP等激發
3.2激發波長 561 nm，用于PE, mCherry等激發
3.3激發波長 640 nm，用于APC, Cy5等激發 
3.4 探測通道 500-550 nm，用于探測 FITC, GFP等
3.5探測通道570-610nm，用于探測PE, mCherry等 
3.6探測通道665-735 nm，用于探測,APC, Cy5等
起始工具包
可用于100個實驗，包括：
 -樣品注射器，針頭，連接管
 -  100 FlicXX（可選擇通道尺寸：15,20,30或40μm）
 -  120毫升CellCarrier（可選擇粘度：高或低）

代表文獻：
[1] High-throughput assessment of mechanical properties of stem cell derived red blood cells, toward cellular downstream processing. Scientific Reports 2017. Guzniczak E., Mohammad Zadeh m., Dempsey F., Jimenez M., Bock H., Whyte G., Willoughby N. & Bridle H.
[2] Harnessing the adaptive potential of mechanoresponsive proteins to overwhelm pancreatic cancer dissemination and invasion. BioRxiv. preprint. 2017
Surcel A., Schiffhauer E.S., Thomas D., Zhu Q., DiNapoli K., Herbig M., Otto O., Guck J., Jaffee E., Iglesias P., Anders R., Robinson D.
[3] Real-time fluorescence and deformability cytometry - flow cytometry goes mechanics. BioRxiv. preprint. 2017. Rosendahl P., Plak K., Jacobi A., Kraeter M., Toepfner N., Otto O., Herold C., Winzi M., Herbig M., Ge Y., Girardo S., Wagner K., Baum B., Guck J.
[4] Detection Of Human Disease Conditions By Single-Cell Morpho-Rheological Phenotyping Of Whole Blood. BioRxiv. preprint. 2017. Töpfner N., Herold C., Otto O., Rosendahl P., Jacobi A., Kräter M., Stächele J., Menschner L., Herbig M., Ciuffreda L., Ranford-Cartwright L., Grzybek M., Coskun U., Reithuber E., Garriss G., Mellroth P., Henriques-Normark B., Tregay N., Suttorp M., Bornhäuser M., Chilvers E.R., Berner R., Guck J.
[5] Toxicity and Immunogenicity in Murine Melanoma following Exposure to Physical Plasma-Derived Oxidants. Oxidative Medicine and Cellular Longevity. 2017. Bekeschus S., Rödder K., Fregin B., Otto O., Lippert M., Weltmann KD., Wende K., Schmidt A., Gandhirajan RK.
[6] Numerical Simulation of Real-Time Deformability Cytometry To Extract Cell Mechanical Properties. ACS Biomater. Sci. Eng. 2017. Mokbel M., Mokbel D., Mietke A., Träber N., Girardo S., Otto O., Guck J., and Aland S.
[7] Actin stress fiber organization promotes cell stiffening and proliferation of pre-invasive breast cancer cells. Nature Communications. 2017. Tavares S., Vieira AF., Taubenberger AV., Araújo M., Martins NP., Brás-Pereira C., Polónia A., Herbig M., Barreto C., Otto O., Cardoso J., Pereira-Leal JB., Guck J., Paredes J., Janody F.
[8] High-throughput cell mechanical phenotyping for label-free titration assays of cytoskeletal modifications. Cytoskeleton. 2017. Golfier S., Rosendahl P., Mietke A., Herbig M., Guck J., Otto O.
[9] Mapping of Deformation to Apparent Young's Modulus in Real-Time Deformability Cytometry arXiv.org. 2017 Herold C.
[10] Plasmodium falciparum erythrocyte-binding antigen 175 triggers a biophysical change in the red blood cell that facilitates invasion. PNAS. 2017. Koch M., Wright KE., Otto O., Herbig M., Salinas ND., Tolia NH., Satchwell TJ., Guck J., Brooks NJ., Baum J.
[11] Initiation of acute graft-versus-host disease by angiogenesis. Blood. 2017. Riesner K, Shi Y, Jacobi A, Kraeter M, Kalupa M, McGearey A, Mertlitz S, Cordes S, Schrezenmeier JF, Mengwasser J, Westphal S, Perez-Hernandez D, Schmitt C, Dittmar G, Guck J, Penack O.
[12] V-ATPase inhibition increases cancer cell stiffness and blocks membrane related Ras signaling - a new option for HCC therapy. Oncotarget. 2016. Bartel K, Winzi M, Ulrich M, Koeberle A, Menche D, Werz O, Müller R, Guck J, Vollmar AM, von Schwarzenberg K.
[13] The F-actin modifier villin regulates insulin granule dynamics and exocytosis downstream of islet cell autoantigen 512. Mol Metab. 2016. Mziaut H, Mulligan B, Hoboth P, Otto O, Ivanova A, Herbig M, Schumann D, Hildebrandt T, Dehghany J, Sönmez A, Münster C, Meyer-Hermann M, Guck J, Kalaidzidis Y, Solimena M.
[14] pH-driven transition of the cytoplasm from a fluid- to a solid-like state promotes entry into dormancy. eLife 2016. M. C. Munder, D. Midtvedt, T. Franzmann, E. Nüske, O. Otto, M. Herbig, E. Ulbricht, P. Müller, A. Taubenberger, S. Maharana, L. Malinovska, D. Richter, J. Guck, V. Zaburdaev and S. Alberti. A
[15] Mechanical phenotyping of primary human skeletal stem cells in heterogeneous populations by real-time deformability cytometry. Integrative Biology 2016. M. Xavier, P. Rosendahl, M. Herbig, M. Kräter, D. Spencer, M. Bornhäuser, R. O. C. Oreffo, H. Morgan, J. Guck and O. Otto.
[16] Myosin II Activity Softens Cells in Suspension. Biophysical Journal 2015. C. J. Chan, A. E. Ekpenyong, S. Golfier, W. Li, K. J. Chalut, O. Otto, J. Elgeti, J. Guck and F. Lautenschläger.
[17] Association of the EGF-TM7 receptor CD97 expression with FLT3-ITD in acute myeloid leukemia. Oncotarget 2015. M. Wobus, M. Bornhäuser, J. Guck, O. Otto, A. Jacobi, M. Kräter, C. Ortlepp, G. Ehninger, Ch. Thiede, and U. Oelschlägel.
[18] Extracting Cell Stiffness from Real-Time Deformability Cytometry: Theory and Experiment. Biophysical Journal 2015. A. Mietke, O. Otto, S. Girardo, P. Rosendahl, A. Taubenberger, S. Golfier, E. Ulbricht, S. Aaland, J. Guck and E. Fischer-Friedrich.
[19] Cell Mechanics: Combining Speed with Precision. Biophysical Journal 2015. Hans M. Wyss
[20] Real-time deformability cytometry: on-the-fly cell mechanical phenotyping. Nature Methods 2015. O. Otto, Ph. Rosendahl, A. Mietke, S. Golfier, Ch. Herold, D. Klaue, S. Girardo, S. Pagliara, A. Ekpenyong, A. Jacobi, M. Wobus, N. Töpfner, U. F. Keyser, J. Mansfeld, E. Fischer-Friedrich, and J. Guck
[21] Mechanics Meets Medicine Science Translational Medicine 2013. Jochen Guck and Edwin R. Chilvers.