高通量單細(xì)胞力學(xué)熒光測(cè)試分析系統(tǒng)將細(xì)胞生物力學(xué)特性同流式細(xì)胞儀有機(jī)的結(jié)合起來，能夠在單細(xì)胞水平下，高通量、無生物標(biāo)記物的條件下，快速研究細(xì)胞的生物力學(xué)特性。

為了給細(xì)胞加載力學(xué)刺激，單個(gè)細(xì)胞被泵驅(qū)動(dòng)通過橫截面略大于細(xì)胞的橫截面的微通道。細(xì)胞周圍的流體的壓力梯度創(chuàng)造出一個(gè)流動(dòng)剖面而影響細(xì)胞的水動(dòng)力學(xué)。通過流體的流速和粘度可控制作用于細(xì)胞上的力。
細(xì)胞可以通過水動(dòng)力可使細(xì)胞發(fā)生形變；
力由流體的流速和粘度控制；
軟細(xì)胞能展示出更大程度的變形。

產(chǎn)品配置列表
高通量單細(xì)胞力學(xué)特性分析模塊
包括：
 - 高速成像系統(tǒng)（CMOS相機(jī)和圖像采集卡）
 - 大功率LED照明系統(tǒng)（460 nm）
 - 帶兩個(gè)注射器模塊的精密注射泵
 - 泵架，SampleStage和SampleBox，
 - 高性能計(jì)算機(jī)
 - 控制和采集軟件（單機(jī)許可證，附帶許可協(xié)議）
- 用于后期處理的數(shù)據(jù)可視化軟件
倒置顯微鏡 - 蔡司Axio Observer 3
和高通量單細(xì)胞力學(xué)特性分析模塊一起進(jìn)行“實(shí)時(shí)高通量細(xì)胞力學(xué)性能測(cè)試”。
包含：
 - 左側(cè)端口，與高速相機(jī)實(shí)現(xiàn)高速通信
 -  40x物鏡，NA 0.65，空氣/無浸泡
 - 手動(dòng)XY平臺(tái)
2.1 附件選項(xiàng)1：升級(jí)到自動(dòng) XY 平臺(tái)
2.2附件選項(xiàng)2：升級(jí)到 Axio Observer 7 和自動(dòng)XY 平臺(tái)
保溫模塊（選配）
與蔡司 Axio Observer兼容，加熱高通量單細(xì)胞力學(xué)特性分析模塊，控制樣品在測(cè)試期間的溫度在室溫和37°C之間。
熒光模塊（選配）
在單細(xì)胞力學(xué)測(cè)量同時(shí)，進(jìn)行熒光強(qiáng)度測(cè)量。
與蔡司Axio Observer顯微鏡兼容，激光發(fā)射模塊直接接到顯微鏡側(cè)面端口上。 
可選擇的激發(fā)波長(zhǎng)和檢測(cè)通道如下：
3.1 激發(fā)波長(zhǎng) 488nm，用于FITC，GFP等激發(fā)
3.2激發(fā)波長(zhǎng) 561 nm，用于PE, mCherry等激發(fā)
3.3激發(fā)波長(zhǎng) 640 nm，用于APC, Cy5等激發(fā) 
3.4 探測(cè)通道 500-550 nm，用于探測(cè) FITC, GFP等
3.5探測(cè)通道570-610nm，用于探測(cè)PE, mCherry等 
3.6探測(cè)通道665-735 nm，用于探測(cè),APC, Cy5等
起始工具包
可用于100個(gè)實(shí)驗(yàn)，包括：
 -樣品注射器，針頭，連接管
 -  100 FlicXX（可選擇通道尺寸：15,20,30或40μm）
 -  120毫升CellCarrier（可選擇粘度：高或低）

代表文獻(xiàn)：
[1] High-throughput assessment of mechanical properties of stem cell derived red blood cells, toward cellular downstream processing. Scientific Reports 2017. Guzniczak E., Mohammad Zadeh m., Dempsey F., Jimenez M., Bock H., Whyte G., Willoughby N. & Bridle H.
[2] Harnessing the adaptive potential of mechanoresponsive proteins to overwhelm pancreatic cancer dissemination and invasion. BioRxiv. preprint. 2017
Surcel A., Schiffhauer E.S., Thomas D., Zhu Q., DiNapoli K., Herbig M., Otto O., Guck J., Jaffee E., Iglesias P., Anders R., Robinson D.
[3] Real-time fluorescence and deformability cytometry - flow cytometry goes mechanics. BioRxiv. preprint. 2017. Rosendahl P., Plak K., Jacobi A., Kraeter M., Toepfner N., Otto O., Herold C., Winzi M., Herbig M., Ge Y., Girardo S., Wagner K., Baum B., Guck J.
[4] Detection Of Human Disease Conditions By Single-Cell Morpho-Rheological Phenotyping Of Whole Blood. BioRxiv. preprint. 2017. Töpfner N., Herold C., Otto O., Rosendahl P., Jacobi A., Kräter M., Stächele J., Menschner L., Herbig M., Ciuffreda L., Ranford-Cartwright L., Grzybek M., Coskun U., Reithuber E., Garriss G., Mellroth P., Henriques-Normark B., Tregay N., Suttorp M., Bornhäuser M., Chilvers E.R., Berner R., Guck J.
[5] Toxicity and Immunogenicity in Murine Melanoma following Exposure to Physical Plasma-Derived Oxidants. Oxidative Medicine and Cellular Longevity. 2017. Bekeschus S., Rödder K., Fregin B., Otto O., Lippert M., Weltmann KD., Wende K., Schmidt A., Gandhirajan RK.
[6] Numerical Simulation of Real-Time Deformability Cytometry To Extract Cell Mechanical Properties. ACS Biomater. Sci. Eng. 2017. Mokbel M., Mokbel D., Mietke A., Träber N., Girardo S., Otto O., Guck J., and Aland S.
[7] Actin stress fiber organization promotes cell stiffening and proliferation of pre-invasive breast cancer cells. Nature Communications. 2017. Tavares S., Vieira AF., Taubenberger AV., Araújo M., Martins NP., Brás-Pereira C., Polónia A., Herbig M., Barreto C., Otto O., Cardoso J., Pereira-Leal JB., Guck J., Paredes J., Janody F.
[8] High-throughput cell mechanical phenotyping for label-free titration assays of cytoskeletal modifications. Cytoskeleton. 2017. Golfier S., Rosendahl P., Mietke A., Herbig M., Guck J., Otto O.
[9] Mapping of Deformation to Apparent Young's Modulus in Real-Time Deformability Cytometry arXiv.org. 2017 Herold C.
[10] Plasmodium falciparum erythrocyte-binding antigen 175 triggers a biophysical change in the red blood cell that facilitates invasion. PNAS. 2017. Koch M., Wright KE., Otto O., Herbig M., Salinas ND., Tolia NH., Satchwell TJ., Guck J., Brooks NJ., Baum J.
[11] Initiation of acute graft-versus-host disease by angiogenesis. Blood. 2017. Riesner K, Shi Y, Jacobi A, Kraeter M, Kalupa M, McGearey A, Mertlitz S, Cordes S, Schrezenmeier JF, Mengwasser J, Westphal S, Perez-Hernandez D, Schmitt C, Dittmar G, Guck J, Penack O.
[12] V-ATPase inhibition increases cancer cell stiffness and blocks membrane related Ras signaling - a new option for HCC therapy. Oncotarget. 2016. Bartel K, Winzi M, Ulrich M, Koeberle A, Menche D, Werz O, Müller R, Guck J, Vollmar AM, von Schwarzenberg K.
[13] The F-actin modifier villin regulates insulin granule dynamics and exocytosis downstream of islet cell autoantigen 512. Mol Metab. 2016. Mziaut H, Mulligan B, Hoboth P, Otto O, Ivanova A, Herbig M, Schumann D, Hildebrandt T, Dehghany J, Sönmez A, Münster C, Meyer-Hermann M, Guck J, Kalaidzidis Y, Solimena M.
[14] pH-driven transition of the cytoplasm from a fluid- to a solid-like state promotes entry into dormancy. eLife 2016. M. C. Munder, D. Midtvedt, T. Franzmann, E. Nüske, O. Otto, M. Herbig, E. Ulbricht, P. Müller, A. Taubenberger, S. Maharana, L. Malinovska, D. Richter, J. Guck, V. Zaburdaev and S. Alberti. A
[15] Mechanical phenotyping of primary human skeletal stem cells in heterogeneous populations by real-time deformability cytometry. Integrative Biology 2016. M. Xavier, P. Rosendahl, M. Herbig, M. Kräter, D. Spencer, M. Bornhäuser, R. O. C. Oreffo, H. Morgan, J. Guck and O. Otto.
[16] Myosin II Activity Softens Cells in Suspension. Biophysical Journal 2015. C. J. Chan, A. E. Ekpenyong, S. Golfier, W. Li, K. J. Chalut, O. Otto, J. Elgeti, J. Guck and F. Lautenschläger.
[17] Association of the EGF-TM7 receptor CD97 expression with FLT3-ITD in acute myeloid leukemia. Oncotarget 2015. M. Wobus, M. Bornhäuser, J. Guck, O. Otto, A. Jacobi, M. Kräter, C. Ortlepp, G. Ehninger, Ch. Thiede, and U. Oelschlägel.
[18] Extracting Cell Stiffness from Real-Time Deformability Cytometry: Theory and Experiment. Biophysical Journal 2015. A. Mietke, O. Otto, S. Girardo, P. Rosendahl, A. Taubenberger, S. Golfier, E. Ulbricht, S. Aaland, J. Guck and E. Fischer-Friedrich.
[19] Cell Mechanics: Combining Speed with Precision. Biophysical Journal 2015. Hans M. Wyss
[20] Real-time deformability cytometry: on-the-fly cell mechanical phenotyping. Nature Methods 2015. O. Otto, Ph. Rosendahl, A. Mietke, S. Golfier, Ch. Herold, D. Klaue, S. Girardo, S. Pagliara, A. Ekpenyong, A. Jacobi, M. Wobus, N. Töpfner, U. F. Keyser, J. Mansfeld, E. Fischer-Friedrich, and J. Guck
[21] Mechanics Meets Medicine Science Translational Medicine 2013. Jochen Guck and Edwin R. Chilvers.