Pall Xpansion貼壁細(xì)胞生物反應(yīng)器
——一次性全封閉生物反應(yīng)器，間充質(zhì)干細(xì)胞培養(yǎng)，種子細(xì)胞培養(yǎng)

 
美國Pall頗爾公司生產(chǎn)的Xpansion系列碟片式生物反應(yīng)器（Xpansion多層板生物反應(yīng)器，Xpansion Multiplate Bioreactor System），專為對(duì)剪切力敏感、粘附的哺乳動(dòng)物細(xì)胞設(shè)計(jì)，特別適用于貼壁干細(xì)胞的培養(yǎng)，如間充質(zhì)干細(xì)胞等，以及種子培養(yǎng)。通過緊湊的多層碟片結(jié)構(gòu)，Xpansion生物反應(yīng)器可提供高達(dá)122,400cm²的細(xì)胞培養(yǎng)面積，并提供全自動(dòng)的氣體擴(kuò)散系統(tǒng)、一次性的pH、DO、溫度傳感器和攪拌裝置，實(shí)時(shí)監(jiān)控培養(yǎng)pH、溶氧、二氧化碳等數(shù)據(jù)，滿足cGMP生產(chǎn)要求。
 


 
Xpansion碟片采用親水聚苯乙烯材料，符合美國藥典VI級(jí)標(biāo)準(zhǔn)，多個(gè)碟片疊加，形成的中央柱結(jié)構(gòu)用于氣體交換，并通過磁力葉輪攪拌促使培養(yǎng)基在各層碟片中均勻流動(dòng)。
 


 
Xpansion碟片高度緊密排列，占地面積小，一個(gè)Xpansion 200生物反應(yīng)器（高度僅0.8m），可提供與20個(gè)10層細(xì)胞工廠（高度約4m）相當(dāng)?shù)呐囵B(yǎng)表面積，節(jié)省80%空間。而且可以從傳統(tǒng)細(xì)胞培養(yǎng)耗材，直接轉(zhuǎn)換為Xpansion進(jìn)行培養(yǎng)，工藝轉(zhuǎn)換簡單。
 



Xpansion生物反應(yīng)器優(yōu)勢(shì)

①符合美國藥典VI級(jí)的原材料
②一次性使用的封閉式生物反應(yīng)器，降低污染風(fēng)險(xiǎn)
③與傳統(tǒng)培養(yǎng)器皿相似的化學(xué)性質(zhì)表面，可輕松適應(yīng)培養(yǎng)體系的轉(zhuǎn)換
④對(duì)培養(yǎng)條件實(shí)時(shí)監(jiān)控，提高實(shí)驗(yàn)重復(fù)性，可追溯，滿足批次間一致性、合規(guī)性
⑤二維多層設(shè)計(jì)，培養(yǎng)板碟片層間距纖薄，極大程度節(jié)約空間
⑥減少操作，流程簡單
⑦多種型號(hào)，滿足不同工藝尺寸和擴(kuò)大規(guī)模



 
參考文獻(xiàn)

1. Scale-up of hepatic progenitor cells from multitray stack to 2-D bioreactors. Matthieu Egloff, Florence Collignon, Jean-Franois Michiels, et al. Springer, Article number: P61 (2013) 

2. Utilizing a Multiplate Bioreactor to Preserve the Integrity of the Culture. Jean-Franois Michiels and Matthieu Egloff. Genetic Engineering & Biotechnology News, 2013, VOL.33, NO.2

3. A new cell therapy sector arising from the convergence of cell and gene therapy. E.J. Culme-Seymour, S. Edwards-Parton, J. Carmen, et al.Cytotherapy, 2013, Vol 15, Iss 4

4. IGF-1 and HGF Preconditioning of Bone Marrow Mesenchymal Stem Cells Reduced Proteinuria in Rat Focal Segmental Glomerulosclerosis Model.A. O.B.Sahan, I.Onbasilar, K.S.Gucer, et al. Cytotherapy, 2020, Volume 22, Issue 5, Page S100

5. Assessment of Pall's Xpansion bioreactor for the production of mesenchymal stromal cells for therapeutic use. H.Rouard, J.Kadoch, M.Lecuyer, et al. Cytotherapy, 2020, Volume 22, Issue 5, Pages S99-S100

6. Inverse modeling of geochemical and mechanical compaction in sedimentary basins through Polynomial Chaos Expansion. G. Porta, L. Tamellini, V. Lever, M. Riva. Water Resources Research, 2014, Volume 50, Issue 12, Pages 9414-9431

7. Pall Xpansion Bioreactor Supports Progenitor Cell Growth to >1 Million cells/cm2 and Proper Cell Differentiation. A.Laskowski, S.Gupta. Cytotherapy, 2020, Volume 22, Issue 5, Page S113

8. Overcoming adherent seed train biomass limitations: Pall Xpansion bioreactor. M.Olson, L.Truong, O.Becheau, T.Sanderson. Cytotherapy, 2020, Volume 22, Issue 5, Pages S185-S186

9. Platform approach for the AAV purification process including membrane chromatography for polishing. K.Boenning, J.Huato, A.Hejmowski, et al. Cytotherapy, 2020, Volume 22, Issue 5, Page S186

10. A rapid and reliable qPCR based system to quantitate HEK 293 residual DNA for GMP lot release in gene therapy manufacturing. L.Yan, M.Mateling, K.Norman. Cytotherapy, 2020, Volume 22, Issue 5, Page S186
 



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