Pall Xpansion貼壁細胞生物反應器
——一次性全封閉生物反應器，間充質干細胞培養，種子細胞培養

 
美國Pall頗爾公司生產的Xpansion系列碟片式生物反應器（Xpansion多層板生物反應器，Xpansion Multiplate Bioreactor System），專為對剪切力敏感、粘附的哺乳動物細胞設計，特別適用于貼壁干細胞的培養，如間充質干細胞等，以及種子培養。通過緊湊的多層碟片結構，Xpansion生物反應器可提供高達122,400cm²的細胞培養面積，并提供全自動的氣體擴散系統、一次性的pH、DO、溫度傳感器和攪拌裝置，實時監控培養pH、溶氧、二氧化碳等數據，滿足cGMP生產要求。
 


 
Xpansion碟片采用親水聚苯乙烯材料，符合美國藥典VI級標準，多個碟片疊加，形成的中央柱結構用于氣體交換，并通過磁力葉輪攪拌促使培養基在各層碟片中均勻流動。
 


 
Xpansion碟片高度緊密排列，占地面積小，一個Xpansion 200生物反應器（高度僅0.8m），可提供與20個10層細胞工廠（高度約4m）相當的培養表面積，節省80%空間。而且可以從傳統細胞培養耗材，直接轉換為Xpansion進行培養，工藝轉換簡單。
 



Xpansion生物反應器優勢

①符合美國藥典VI級的原材料
②一次性使用的封閉式生物反應器，降低污染風險
③與傳統培養器皿相似的化學性質表面，可輕松適應培養體系的轉換
④對培養條件實時監控，提高實驗重復性，可追溯，滿足批次間一致性、合規性
⑤二維多層設計，培養板碟片層間距纖薄，極大程度節約空間
⑥減少操作，流程簡單
⑦多種型號，滿足不同工藝尺寸和擴大規模



 
參考文獻

1. Scale-up of hepatic progenitor cells from multitray stack to 2-D bioreactors. Matthieu Egloff, Florence Collignon, Jean-Franois Michiels, et al. Springer, Article number: P61 (2013) 

2. Utilizing a Multiplate Bioreactor to Preserve the Integrity of the Culture. Jean-Franois Michiels and Matthieu Egloff. Genetic Engineering & Biotechnology News, 2013, VOL.33, NO.2

3. A new cell therapy sector arising from the convergence of cell and gene therapy. E.J. Culme-Seymour, S. Edwards-Parton, J. Carmen, et al.Cytotherapy, 2013, Vol 15, Iss 4

4. IGF-1 and HGF Preconditioning of Bone Marrow Mesenchymal Stem Cells Reduced Proteinuria in Rat Focal Segmental Glomerulosclerosis Model.A. O.B.Sahan, I.Onbasilar, K.S.Gucer, et al. Cytotherapy, 2020, Volume 22, Issue 5, Page S100

5. Assessment of Pall's Xpansion bioreactor for the production of mesenchymal stromal cells for therapeutic use. H.Rouard, J.Kadoch, M.Lecuyer, et al. Cytotherapy, 2020, Volume 22, Issue 5, Pages S99-S100

6. Inverse modeling of geochemical and mechanical compaction in sedimentary basins through Polynomial Chaos Expansion. G. Porta, L. Tamellini, V. Lever, M. Riva. Water Resources Research, 2014, Volume 50, Issue 12, Pages 9414-9431

7. Pall Xpansion Bioreactor Supports Progenitor Cell Growth to >1 Million cells/cm2 and Proper Cell Differentiation. A.Laskowski, S.Gupta. Cytotherapy, 2020, Volume 22, Issue 5, Page S113

8. Overcoming adherent seed train biomass limitations: Pall Xpansion bioreactor. M.Olson, L.Truong, O.Becheau, T.Sanderson. Cytotherapy, 2020, Volume 22, Issue 5, Pages S185-S186

9. Platform approach for the AAV purification process including membrane chromatography for polishing. K.Boenning, J.Huato, A.Hejmowski, et al. Cytotherapy, 2020, Volume 22, Issue 5, Page S186

10. A rapid and reliable qPCR based system to quantitate HEK 293 residual DNA for GMP lot release in gene therapy manufacturing. L.Yan, M.Mateling, K.Norman. Cytotherapy, 2020, Volume 22, Issue 5, Page S186
 



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——Pall一級代理商，北京澤平