Preparation :

Produced in Sprague Dawley rats using highly pure (>98%) recombinant murine IL-10 as the immunizing antigen. This IgG2_A antibody was purified from cell culture by Protein G affinity chromatography.

Catalog #:

500-M128

Source :

Rats

Formulation :

A sterile filtered antibody solution was lyophilized from PBS.

Stability :

The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20^o C. Frozen aliquots are stable for at least six weeks at 2-8^o C or for at least six months at -20^o C. Avoid repeated freeze-thaw cycles.

Reconstitution:

Centrifuge vial prior to opening. Reconstitute in sterile water to a concentration of 1.0 mg/ml.

Applications :

Neutralization 

To yield one-half maximal inhibition [ND ₅₀ ] of the biological activity of mIL-10 (0.5 ng/ml), a concentration of 3.0-5.0 μg/ml of this antibody is required.

Elisa 

In a sandwich ELISA (assuming 100µl/well), a concentration of 4.0-8.0 µg/ml of this antibody will detect at least 1600 pg/ml of recombinant murine IL-10 when used with 's biotinylated antigen affinity purified anti-murine IL-10 (500-P60Bt) as the detection antibody at a concentration of approximately 0.5-1.0 µg/ml.

Western Blot 

To detect mIL-10 by Western Blot analysis this antibody can be used at a concentration of 1.0-2.0 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant mIL-10 is 2.0-4.0 ng/lane, under reducing or non-reducing conditions.

Country of Origin :

USA

Special Sizes:
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Usage:
For Research Use Only. Not for use in diagnostic or therapeutic procedures.