Synonyms :

Endoproteinase Lys/Arg-Arg

Description :

Proteases (also called Proteolytic Enzymes, Peptidases, or Proteinases) are enzymes that hydrolyze the amide bonds within proteins or peptides. Most proteases act in a specific manner, hydrolyzing bonds at or adjacent to specific residues or a specific sequence of residues contained within the substrate protein or peptide. Proteases play an important role in most diseases and biological processes including prenatal and postnatal development, reproduction, signal transduction, the immune response, various autoimmune and degenerative diseases, and cancer. They are also an important research tool, frequently used in the analysis and production of proteins. Kex-2 cleaves at the carboxyl end of the recognition sequences Arg-Arg/X and Lys-Arg/X. Recombinant Yeast Kex-2 is a 59.9 kDa protease consisting of 554 amino acid residues.

Catalog #:

450-45

Source :

(BTI-Tn-5B1-4) High-5 Insect Cells*

Purity :

Greater than 95% by SDS-PAGE gel and HPLC analyses.

Endotoxin Level :

Endotoxin level is less than 0.1 ng per μg (1EU/μg).

Biological Activity :

Recombinant Kex-2 from High-5 insect cells contains the same specific activity and recognition sequence specificity as yeast derived KEX-2. 1 milligram of recombinant KEX-2 contains activity equivalent to at least 40 units of yeast derived KEX-2. Cleaves at the carboxyl side of K/R-R.

AA Sequence :

APPMDSSLLP VKEAEDKLSI NDPLFERQWH LVNPSFPGSD INVLDLWYNN ITGAGVVAAI VDDGLDYENE DLKDNFCAEG SWDFNDNTNL PKPRLSDDYH GTRCAGEIAA KKGNNFCGVG VGYNAKISGI RILSGDITTE DEAASLIYGL DVNDIYSCSW GPADDGRHLQ GPSDLVKKAL VKGVTEGRDS KGAIYVFASG NGGTRGDNCN YDGYTNSIYS ITIGAIDHKD LHPPYSEGCS AVMAVTYSSG SGEYIHSSDI NGRCSNSHGG TSAAAPLAAG VYTLLLEANP NLTWRDVQYL SILSAVGLEK NADGDWRDSA MGKKYSHRYG FGKIDAHKLI EMSKTWENVN AQTWFYLPTL YVSQSTNSTE ETLESVITIS EKSLQDANFK RIEHVTVTVD IDTEIRGTTT VDLISPAGII SNLGVVRPRD VSSEGFKDWT FMSVAHWGEN GVGDWKIKVK TTENGHRIDF HSWRLKLFGE SIDSSKTETF VFGNDKEEVE PAATESTVSQ YSASSTSISI SATSTSSISI GVETSAIPQT TTASTDPDSD PNTP

PubMed Link :

Kex2

Country of Origin :

USA

Special Sizes:
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Usage:
For Research Use Only. Not for use in diagnostic or therapeutic procedures.